Affiliation:
1. Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
2. Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Abstract
A system has been developed to identify 11 genetic markers associated with the risk of obesity: rs10852521, rs11075990, rs1121980, rs1421085, rs1477196, rs17817449, rs3751812, rs7206790, rs8047395, rs9940128 (FTO gene) and rs1137101 (LEPR gene) by minisequencing (SNaPshot analysis). The conditions for carrying out the amplification and minisequencing reactions, as well as the compositions of the reaction mixtures, were optimized so that the analysis was carried out for all 11 markers simultaneously. The resulting system was tested and showed a high degree of reproducibility and sensitivity required for the detection of these polymorphisms.
Publisher
Publishing House Belorusskaya Nauka
Subject
Inorganic Chemistry,Organic Chemistry,Chemistry (miscellaneous),Analytical Chemistry
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