ENZYME IMMUNOASSAY KIT FOR THE DETERMINATION OF DEOXYNIVALENOL

Author:

Vashkevich I. I.,Kuprienko O. S.,Gorbachova I. V.,Yastrebova A. A.,Terentieva T. V.,Kornilovich G. S.,Sukhenko L. N.,Shibeko A. I.,Sviridov O. V.

Abstract

A reagent kit EIA-DEOXYNIVALENOL for the determination of mycotoxin deoxynivalenol (DON) in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The basic components of the kit are polyclonal antibodies to DON, obtained as a result of immunization of rabbits with a conjugate of DON with bovine serum albumin and a conjugate of horseradish peroxidase with DON. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of DON content of agricultural products in a range of 0.2 to 6.0 mg/kg with proper accuracy and precision. The limit of quantitative determination of DON in grain and cereal foods does not exceed 0.2 mg/kg.

Publisher

Publishing House Belorusskaya Nauka

Subject

Inorganic Chemistry,Organic Chemistry,Chemistry (miscellaneous),Analytical Chemistry

Reference13 articles.

1. Mirzoev D. M., Razokov S. I., Sattorov S. S., Sulaymon H. N., Kononenko G. P., Burkin A. N. Mikotoksiny, zagryaznyayuschie korma i pischevye produkty. Kishovarz [Kishovarz], 2014, no. 2, pp. 31–32 (in Russian).

2. Ahmadyishin R. A., Kanarskii A. V., Kanarskaya Z. A. Mycotoxins are food contaminants. Vestnik Kazanskogo tehnologicheskogo universiteta = Herald of Kazan Technological University, 2007, no. 2, pp. 88–103 (in Russian).

3. Fisinin V. I., Surai P. F. Properties and toxicity of deoxynivalenol. Zhivotnovodstvo Rossii [Сattle breeding in Russia], 2012, no. 5, pp. 11–14 (in Russian).

4. Maragos C. M., McCormick S. P. Monoclonal Antibodies for the Mycotoxins Deoxynivalenol and 3-Acetyl-Deoxynivalenol. Food and Agricultural. Immunology, 2000, vol. 12, no 3 pp. 181–192. https://doi. org/10.1080/09540100050140722

5. Casale W. L., Pestka J. J., Hart L. P. Enzyme-linked immunosorbent assay employing monoclonal antibody specific for deoxynivalenol (vomitoxin) and several analogs. Journal of Agricultural and Food Chemistry, 1988, vol. 36, no. 3, pp. 663–668. https://doi.org/10.1021/jf00081a064

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Direct competitive enzyme immunoassay method for the determination of ochratoxin a in feeds and foods;Proceedings of the National Academy of Sciences of Belarus, Chemical Series;2019-03-13

2. ENZYME IMMUNOASSAY KIT FOR THE DETERMINATION OF DEOXYNIVALENOL;Proceedings of the National Academy of Sciences of Belarus, Chemical Series;2018-09-16

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3