A plasmid system for optimization of Fab′ production in Escherichia coli: importance of balance of heavy chain and light chain synthesis

Author:

Humphreys David P,Carrington Bruce,Bowering Leigh C,Ganesh Ravindra,Sehdev Mukesh,Smith Bryan J,King Lloyd M,Reeks Dominic G,Lawson Alastair,Popplewell Andrew G

Publisher

Elsevier BV

Subject

Biotechnology

Reference17 articles.

1. Therapeutic antibody production technologies: molecules, applications, expression, and purification;Humphreys;Curr. Opin. Drug Disc. Dev.,2001

2. Pharmacokinetics of monoclonal immunoglobulins G1, F(ab′)2, and Fab′ in mice;Covell;Cancer Res.,1986

3. F(ab′)2 molecules made from E. coli produced Fab′ with hinge sequences conferring increased serum permanence times in an animal model;Humphreys;J. Immunol. Meth.,1998

4. Therapeutic antibody fragments with prolonged in vivo half-lives;Chapman;Nat. Biotech.,1999

5. Preparation of Fcγ for addition to sulfhydryl-expressing ligands with minimal disturbance of the hinge;Stevenson;J. Immunol. Methods,1999

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