Pectinatus brassicae sp. nov., a Gram-negative, anaerobic bacterium isolated from salty wastewater

Abstract

A novel Gram-negative, non-spore-forming, strictly anaerobic, heterotrophic bacterium, strain TYT, was isolated from salty pickle wastewater. Cells were rod-shaped with comb-like flagella, slightly curved and very variable in length. Optimal growth occurred at 28 °C and pH 6.5. Cells were resistant to up to 50 g NaCl l−1. Strain TYT produced acid from glycerol, sucrose, glucose, fructose and mannitol. The main fermentation products from glucose were acetic and propionic acids. Tests for acid phosphatase and naphthol-AS-BI-phosphohydrolase activities were positive. The major fatty acids were C14 : 0 DMA (18.7 %), C15 : 0 (15.4 %), anteiso-C18 : 1 (15.2 %), C11 : 0 (13.3 %) and summed feature 5 (C17 : 1ω7c and/or C17 : 2) (11.0 %). The DNA G+C content was 35.9 mol%. 16S rRNA gene sequence-based phylogenetic analysis indicated that strain TYT represented a novel species of the genus Pectinatus (sequence similarity to other members of the genus ranged from 93.2 to 94.8 %). Based on its phenotypic, genotypic and phylogenetic characteristics, strain TYT is proposed to represent a novel species, named Pectinatus brassicae sp. nov. (type strain TYT  = JCM 17499T  = DSM 24661T).