Efficient transformation of Lactobacillus sake by electroporation-Reference-Cited by-同舟云学术

Efficient transformation of Lactobacillus sake by electroporation

Published:1996-05-01 Issue:5 Volume:142 Page:1273-1279
ISSN:1350-0872
Container-title:Microbiology
language:en
Short-container-title:

Author:

Berthier Françoise¹,Zagorec Monique¹,Champomier-Vergès Marie¹,Ehrlich S. D.²,Morel-Deville Françoise¹

Affiliation:

1. Laboratoire de Recherches sur la Viande, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France

2. Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France

Abstract

A procedure to transform intact Lactobacillus sake cells by electroporation was developed through a systematic examination of the effect of changes in various parameters on the transformation efficiency of Lact. sake strain 64F. The most critical factors were found to be the electrical parameters, the composition of washing and electroporation/storage solutions, and the presence of MgCI2 in the expression medium. Under optimal conditions transformation efficiencies up to 107 transformants (μg supercoiled DNA)-1 were obtained. The optimized procedure was successfully applied to other Lact. sake strains and consistently yielded from 104 to 107 transformants (μg supercoiled DNA)-1.

Publisher

Microbiology Society

Subject

Microbiology

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