Affiliation:
1. Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea
Abstract
A Gram-staining-negative, yellow-pigmented, strictly aerobic bacterial strain motile by gliding, designated BS12T, was isolated from a tidal flat at Boseong, Korea. Cells were moderately halotolerant, catalase- and oxidase-positive rods. Growth was observed at 5–40 °C (optimum, 25 °C), at pH 5.5–9.0 (optimum, pH 7.0–7.5) and in the presence of 1–11 % (w/v) NaCl (optimum, 2–4 %). The major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C17 : 1ω9c and anteiso-C15 : 0. The polar lipid pattern comprised phosphatidylethanolamine, four unidentified aminolipids and three unidentified lipids. The G+C content of the genomic DNA was 42.3 mol% and the only respiratory quinone was menaquinone 6. Phylogenetic inference based on 16S rRNA gene sequences showed that strain BS12T formed a tight phyletic lineage with members of the genus
Gramella
. Strain BS12T was most closely related to ‘
Gramella
jeungdoensis’ HMD3159 with sequence similarity of 97.5 %, but the DNA–DNA relatedness value between the two strains was only 22.1±2.2 %. On the basis of phenotypic and molecular features, strain BS12T was shown to represent a novel species of the genus
Gramella
, for which the name
Gramella
aestuarii sp. nov. is proposed. The type strain is BS12T ( = KACC 16188T = JCM 17790T). An emended description of
Gramella echinicola
is also proposed.
Funder
Ministry of Environment, Korea
National Institute of Biological Resources (NIBR
Ministry of Education, Science and Technology
Cooperative Research Program for Agriculture Science & Technology Development
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Reference42 articles.
1. Unique diversity of carotenoid-producing bacteria isolated from Misasa, a radioactive site in Japan;Asker;Appl Microbiol Biotechnol,2007a
2. Zeaxanthinibacter enoshimensis gen. nov., sp. nov., a novel zeaxanthin-producing marine bacterium of the family Flavobacteriaceae, isolated from seawater off Enoshima Island, Japan;Asker;Int J Syst Evol Microbiol,2007b
3. Whole genome analysis of the marine Bacteroidetes ‘Gramella forsetii’ reveals adaptations to degradation of polymeric organic matter;Bauer;Environ Microbiol,2006
4. Family I. Flavobacteriaceae;Bernardet,2011
5. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family;Bernardet;Int J Syst Evol Microbiol,2002
Cited by
15 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献