Gramella sabulilitoris sp. nov., isolated from a marine sand

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, motile by gliding and rod-shaped bacterial strain, designated HSMS-1T, was isolated from a marine sand collected from the Yellow Sea, Republic of Korea, and identified by a polyphasic taxonomic approach. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that HSMS-1T fell within the clade comprising the type strains of species of the genus Gramella . HSMS-1T exhibited 16S rRNA gene sequence similarity values of 99.0 and 98.7 % to the type strains of Gramella echinicola and Gramella sediminilitoris and of 93.3–98.5 % to the type strains of the other species of the genus Gramella . The ANI and dDDH values between HSMS-1T and the type strains of G. echinicola , Gramella gaetbulicola , Gramella forsetii , Gramella salexigens , Gramella portivictoriae and Gramella flava were 72.6–79.3 % and 17.4–22.2 %, respectively. Mean DNA–DNA relatedness value between HSMS-1T and the type strain of G. sediminilitoris was 18 %. HSMS-1T contained MK-6 as the predominant menaquinone and iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0 3-OH and iso-C16 : 0 as the major fatty acids. The major polar lipid of HSMS-1T was phosphatidylethanolamine. The DNA G+C content of HSMS-1T from genomic sequence data was 39.2 %. Distinguishing phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that HSMS-1T is separated from recognized species of the genus Gramella . On the basis of the data presented, strain HSMS-1T is considered to represent a novel species of the genus Gramella , for which the name Gramella sabulilitoris sp. nov. is proposed. The type strain is HSMS-1T(=KACC 19899T=NBRC 113648T).