Bacillus wuyishanensis sp. nov., isolated from rhizosphere soil of a medical plant, Prunella vulgaris

Abstract

A Gram-staining-positive, rod-shaped, endospore-forming, aerobic bacterium (FJAT-17212T) was isolated from the rhizosphere soil of a medical plant, Prunella vulgaris (common selfheal), on the Wuyishan mountain of China. Isolate FJAT-17212T grew at 10–50 °C (optimum 30 °C), pH 5–11 (optimum pH 7) and with 0–6  % (w/v) NaCl (optimum 2  %). Phylogenetic analyses based on 16S rRNA gene sequences showed that isolate FJAT-17212T was a member of the genus Bacillus and was most closely related to Bacillus galactosidilyticus DSM 15595T (97.3  %). DNA–DNA relatedness between isolate FJAT-17212T and B. galactosidilyticus DSM 15595T was low (35.2  % ± 2.3). The diagnostic diamino acid of the peptidoglycan of isolate FJAT-17212T was meso-diaminopimelic acid and the predominant isoprenoid quinone was MK-7 (80.8  %). The major cellular fatty acids were iso-C15 : 0 (35.7  %), anteiso-C15 : 0 (29.8  %), iso-C14 : 0 (9.9  %) and iso-C16 : 0 (9.9  %) and the DNA G+C content was 39.8  mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate FJAT-17212T represents a novel species within the genus Bacillus, for which the name Bacillus wuyishanensis sp. nov. is proposed. The type strain is FJAT-17212T ( = DSM 27848T = CGMCC 1.12709T).