Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin

Abstract

A mesophilic, acidophilic, spore-forming bacterium, strain 105-2T, was isolated from an acidic Sphagnum peat bog in Wisconsin, USA. Strain 105-2T has 16S rRNA gene sequence similarity to Bacillus sporothermodurans DSM 10599T and Bacillus oleronius DSM 9356T of 97·4 and 97·8 %, respectively. The primary lipoquinone is MK-7 and the major fatty acids are 15 : 0 iso, 15 : 0 anteiso and 17 : 0 anteiso. The predominant polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and a glycolipid. The DNA G+C content was found to be 43·2 mol%. The phenotypic, chemotaxonomic and molecular analyses identified strain 105-2T as a novel Bacillus species, for which the name Bacillus acidicola is proposed. The type strain is 105-2T (=DSM 14745T=ATCC BAA-366T=NRRL B-23453T).