Affiliation:
1. Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, The Netherlands
Abstract
The specific pH values of cellular compartments affect virtually all biochemical processes, including enzyme activity, protein folding and redox state. Accurate, sensitive and compartment-specific measurements of intracellular pH (pHi) dynamics in living cells are therefore crucial to the understanding of stress response and adaptation. We used the pH-sensitive GFP derivative ‘ratiometric pHluorin’ expressed in the cytosol and in the mitochondrial matrix of growingSaccharomyces cerevisiaeto assess the variation in cytosolic pH (pHcyt) and mitochondrial pH (pHmit) in response to nutrient availability, respiratory chain activity, shifts in environmental pH and stress induced by addition of sorbic acid. Thein vivomeasurement allowed accurate determination of organelle-specific pH, determining a constant pHcytof 7.2 and a constant pHmitof 7.5 in cells exponentially growing on glucose. We show that pHcytand pHmitare differentially regulated by carbon source and respiratory chain inhibitors. Upon glucose starvation or sorbic acid stress, pHidecrease coincided with growth stasis. Additionally, pHiand growth coincided similarly in recovery after addition of glucose to glucose-starved cultures or after recovery from a sorbic acid pulse. We suggest a relation between pHiand cellular energy generation, and therefore a relation between pHiand growth.
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308 articles.
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