Repeatability of alkaline inorganic phosphate quantification in the skeletal muscle using 31P‐magnetic resonance spectroscopy at 3 T

Author:

Matias Alexs A.1,Serviente Corinna F.12,Decker Stephen T.13,Erol Muhammet Enes14,Giuriato Gaia1456,Le Fur Yann7,Nagarajan Rajakumar89,Bendahan David7,Layec Gwenael189

Affiliation:

1. Department of Kinesiology University of Massachusetts at Amherst Amherst MA USA

2. Diabetes & Metabolism Research Center University of Utah Salt Lake City UT USA

3. The Institute for Applied Life Sciences University of Massachusetts at Amherst Amherst MA USA

4. School of Health and Kinesiology University of Nebraska at Omaha Omaha NE USA

5. Department of Neuroscience, Biomedicine, and Movement Science University of Verona Verona Italy

6. Department of Surgical, Medical and Dental University of Modena and Reggio Emilia Modena Italy

7. Department of Morphological Sciences Related to Transplant, Oncology and Regenerative Medicine University of Modena and Reggio Emilia Modena Italy

8. Centre de Resonance Magnetique Biologique et Medicale, UMR CNRS 6612, Faculté de Médecine de Marseille Marseille France

9. Human Magnetic Resonance Center Institute for Applied Life Sciences University of Massachusetts at Amherst Amherst MA USA

Abstract

AbstractThe detection of a secondary inorganic phosphate (Pi) resonance, a possible marker of mitochondrial content in vivo, using phosphorus magnetic resonance spectroscopy (31P‐MRS), poses technical challenges at 3 Tesla (T). Overcoming these challenges is imperative for the integration of this biomarker into clinical research. To evaluate the repeatability and reliability of measuring resting skeletal muscle alkaline Pi (Pialk) using with 31P‐MRS at 3 T. After an initial set of experiments on five subjects to optimize the sequence, resting 31P‐MRS of the quadriceps muscles were acquired on two visits (~4 days apart) using an intra‐subjects design, from 13 sedentary to moderately active young male and female adults (22 ± 3 years old) within a whole‐body 3 T MR system. Measurement variability attributed to changes in coil position, shimming procedure, and spectral analysis were quantified. 31P‐MRS data were acquired with a 31P/‐proton (1H) dual‐tuned surface coil positioned on the quadriceps using a pulse‐acquire sequence. Test–retest absolute and relative repeatability was analyzed using the coefficient of variation (CV) and intra‐class correlation coefficients (ICC), respectively. After sequence parameter optimization, Pialk demonstrated high intra‐subject repeatability (CV: 10.6 ± 5.4%, ICC: 0.80). Proximo‐distal change in coil position along the length of the quadriceps introduced Pialk quantitation variability (CV: 28 ± 5%), due to magnetic field inhomogeneity with more distal coil locations. In contrast, Pialk measurement variability due to repeated shims from the same muscle volume (0.40 ± 0.09mM; CV: 6.6%), and automated spectral processing (0.37 ± 0.01mM; CV: 2.3%), was minor. The quantification of Pialk in skeletal muscle via surface coil 31P‐MRS at 3 T demonstrated excellent reproducibility. However, caution is advised against placing the coil at the distal part of the quadriceps to mitigate shimming inhomogeneity.

Funder

National Heart, Lung, and Blood Institute

Publisher

Wiley

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