Construction and characterization of a Lactococcus lactis strain deficient in intracellular ClpP and extracellular HtrA proteases

Author:

Cortes-Perez N. G.1,Poquet I.2,Oliveira M.31,Gratadoux J. J.1,Madsen S. M.4,Miyoshi A.3,Corthier G.1,Azevedo V.3,Langella P.1,Bermúdez-Humarán L. G.1

Affiliation:

1. Unité d'Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France

2. Unité des Bactéries Lactiques et Pathogènes Opportunistes, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France

3. Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, Minas Gerais, Brazil

4. Bioneer A/S, Hørsholm, Denmark

Abstract

ALactococcus lactisstrain deficient in both its major proteases, intracellular (ClpP) and extracellular (HtrA), was constructed and characterized. This strain, hereafter calledclpP-htrA, could be obtained only by conjugation between aclpPdonor strain and anhtrArecipient strain in the NZ9000 context, allowing heterologous gene expression under the control of the NICE (nisin-controlled expression) system. TheclpP-htrAdouble mutant showed both higher stress tolerance (e.g. high temperature and ethanol resistance) and higher viability than singleclpPorhtrAmutant strains. In addition, the secretion rate of two heterologous proteins (staphylococcal nuclease Nuc and Nuc-E7) was also higher inclpP-htrAthan in the wild-type strain. This strain should be a useful host for high-level production and quality of stable heterologous proteins.

Publisher

Microbiology Society

Subject

Microbiology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3