Production of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis-Reference-Cited by-同舟云学术

Production of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis

Published:2002-02 Issue:2 Volume:68 Page:917-922
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bermúdez-Humarán L. G.¹²,Langella P.¹,Miyoshi A.¹³,Gruss A.¹,Guerra R. Tamez²,Montes de Oca-Luna R.²,Le Loir Y.¹

Affiliation:

1. Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France

2. Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolas de los Garza, Nuevo León, Mexico

3. Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, Minas Gerais, Brazil

Abstract

ABSTRACT The E7 protein of human papillomavirus type 16 was produced in Lactococcus lactis. Secretion allowed higher production yields than cytoplasmic production. In stationary phase, amounts of cytoplasmic E7 were reduced, while amounts of secreted E7 increased, suggesting a phase-dependent intracellular proteolysis. Fusion of E7 to the staphylococcal nuclease, a stable protein, resulted in a highly stable cytoplasmic protein. This work provides new candidates for development of viral screening systems and for oral vaccine against cervical cancer.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.2.917-922.2002

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