In-vitro assessment of antioxidant and antimicrobial activity of whole porcine-liver hydrolysates and its fractions

Abstract

Aim of the study was to evaluate antioxidant and antimicrobial activity of porcine liver-protein hydrolysates (PLHs) and their fractions recovered from enzymatic hydrolysis. Hydrolysates and fractions were assessed for antioxidants such as 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid, 2,2-diphenyl-1-picrylhydrazyl and ferric-reducing antioxidant power and antimicrobial activity. Inhibition activity for all antioxidant parameters was significantly (P < 0.05) higher for whole PLHs than for its corresponding fractions; however, among their fractions, 5–10 kDa and 1–5 kDa had a relatively higher antioxidant activity than did the other fractions. Trypsin-digested whole PLHs and their fractions exhibited the highest antioxidant and antimicrobial activity, followed by alcalase- and papain-digested PLHs, for all tested microbes. Results accomplished that whole PLHs exhibited better result than its fractions; thus, PLHs can be used as preservatives for food products or utilised for other pharmaceutical purposes.