Natural variation in primary root growth and K+ retention in roots of habanero pepper (Capsicum chinense) under salt stress

Abstract

In this work, we analysed the natural variation in mechanisms for protection against salt stress in pepper varieties (Capsicum chinense Jacq. cv. Rex, Chichen-Itza and Naranja and Capsicum annuum L. cv. Padron), considering primary root growth and viability of the post-stressed seedlings. NaCl-induced K+ and H+ efflux in roots was also studied by ion-selective microelectrodes under application of pharmacological agents. In these pepper varieties, the magnitude of the K+ leakage in the roots positively correlated with growth inhibition of the primary root in the presence of NaCl, with Rex variety showing a higher level of tolerance than Chichen-Itza. The K+ leakage and the activity of the H+ pump in the roots were dependent on the NaCl concentration. Pharmacological analysis indicated that the NaCl-induced K+ leakage was mediated by TEA+-sensitive KOR channels but not by NSCC channels. In addition, we present evidence for the possible participation of proline, and a Na+-insensitive HAK K+ transporter expressed in habanero pepper roots for maintaining K+ homeostasis under salt stress conditions.