Phylogenetic characterization of Salmonella enterica from pig production and humans in Thailand and Laos border provinces
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Published:2019-01
Issue:1
Volume:12
Page:79-84
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ISSN:2231-0916
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Container-title:Veterinary World
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language:en
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Short-container-title:Vet World
Author:
Prathan Rangsiya1, Bitrus Asinamai Athliamai1, Sinwat Nuananong2, Angkititrakul Sunpetch3, Chuanchuen Rungtip1
Affiliation:
1. Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand. 2. Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand; Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University, Kamphaengsean campus, Nakhonpathom, 73140 Thailand. 3. Research Group for Prevention Technology in Livestock, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40000, Thailand.
Abstract
Background and Aim: The genetic relationship among serotypes of Salmonella enterica from food animals, food of animal origin, and human is of interest as the data could provide an important clue for the source of human infection. This study aimed to determine the genetic relatedness of S. enterica from pig production and human in Thailand-Laos border provinces. Materials and Methods: A total of 195 S. enterica serotypes isolated from pig and pork (n=178) and human (n=17) including four serotypes (Typhimurium, Rissen, Derby, and Stanley) were randomly selected to examine their genetic relatedness using highly conserved sequence of three genes (fim A, man B, and mdh). Results: The results showed that 195 Salmonella isolates of four different serotypes were grouped into five different clusters, and members of the same Salmonella serotypes were found in the same cluster. Salmonella isolated from pig production and human in Thailand-Laos border provinces represented overlapping population and revealed a high degree of similarity, indicating close genetic relationship among the isolates. Conclusion: The results support that the determination of Salmonella serotyping combined with analysis of phylogenetic tree can be used track the clonal evolution and genetic diversity of Salmonella serotypes in different host species.
Funder
Thailand Research Fund Chulalongkorn University
Publisher
Veterinary World
Subject
General Veterinary
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