hnRNP C Tetramer Measures RNA Length to Classify RNA Polymerase II Transcripts for Export

Abstract

Choosing the Right Path RNA molecules are synthesized in the cell nucleus, yet many have to be moved to the cytoplasm to be processed and/or to effect their function. Different classes of RNA are transported from the nucleus by different transport systems. Messenger RNAs (mRNAs) and uridine-rich small nuclear RNAs (U snRNAs) are transcribed by RNA polymerase II and are capped and bound by the cap-binding machinery in the nucleus but are exported by different protein complexes. The feature that distinguishes the two classes of RNA is their length: U snRNAs are short and mRNAs are long. Using an in vitro system and human tissue culture cells, McCloskey et al. (p. 1643 ) show that the length of the RNAs is measured by the heterogeneous nuclear ribonicleoprotein (hnRNP) C tetrameric protein complex. The hnRNP C cannot bind to the short U snRNAs, allowing the U snRNA-specific export adaptor protein, PHAX, to bind and mediate export. Longer mRNAs are bound by hnRNP C, which prevents the binding of PHAX, thus identifying these RNAs for export from the nucleus via the mRNA pathway.