Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium

Author:

Mytlis Avishag12ORCID,Kumar Vineet12ORCID,Qiu Tao3ORCID,Deis Rachael12,Hart Neta12,Levy Karine12,Masek Markus45ORCID,Shawahny Amal12,Ahmad Adam12,Eitan Hagai12ORCID,Nather Farouq12,Adar-Levor Shai6ORCID,Birnbaum Ramon Y.6ORCID,Elia Natalie6ORCID,Bachmann-Gagescu Ruxandra45ORCID,Roy Sudipto378ORCID,Elkouby Yaniv M.12ORCID

Affiliation:

1. Department of Developmental Biology and Cancer Research, Hebrew University of Jerusalem Faculty of Medicine, Ein-Kerem Campus, Jerusalem 9112102, Israel.

2. Institute for Medical Research-Israel-Canada (IMRIC), Ein-Kerem Campus, Jerusalem 9112102, Israel.

3. Institute of Molecular and Cell Biology, Proteos 138673, Singapore.

4. Department of Molecular Life Sciences, University of Zurich, 8057 Zurich, Switzerland.

5. Institute of Medical Genetics, University of Zurich, 8952 Schlieren, Switzerland.

6. Departments of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.

7. Department of Biological Sciences, National University of Singapore, 117543 Singapore.

8. Department of Pediatrics, Yong Loo Lin School of Medicine, National University of Singapore, 119288 Singapore.

Abstract

A hallmark of meiosis is chromosomal pairing, which requires telomere tethering and rotation on the nuclear envelope through microtubules, driving chromosome homology searches. Telomere pulling toward the centrosome forms the “zygotene chromosomal bouquet.” Here, we identified the “zygotene cilium” in oocytes. This cilium provides a cable system for the bouquet machinery and extends throughout the germline cyst. Using zebrafish mutants and live manipulations, we demonstrate that the cilium anchors the centrosome to counterbalance telomere pulling. The cilium is essential for bouquet and synaptonemal complex formation, oogenesis, ovarian development, and fertility. Thus, a cilium represents a conserved player in zebrafish and mouse meiosis, which sheds light on reproductive aspects in ciliopathies and suggests that cilia can control chromosomal dynamics.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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