CRISPR-CasΦ from huge phages is a hypercompact genome editor-Reference-Cited by-同舟云学术

CRISPR-CasΦ from huge phages is a hypercompact genome editor

Published:2020-07-17 Issue:6501 Volume:369 Page:333-337
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Pausch Patrick¹²^ORCID,Al-Shayeb Basem¹³^ORCID,Bisom-Rapp Ezra⁴,Tsuchida Connor A.¹⁵^ORCID,Li Zheng⁶^ORCID,Cress Brady F.¹²^ORCID,Knott Gavin J.¹²⁷^ORCID,Jacobsen Steven E.⁶⁸^ORCID,Banfield Jillian F.¹⁹^ORCID,Doudna Jennifer A.¹²⁸¹⁰¹¹^ORCID

Affiliation:

1. Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.

2. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.

3. Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA.

4. College of Natural Resources, University of California, Berkeley, Berkeley, CA, USA.

5. University of California, Berkeley—University of California, San Francisco Graduate Program in Bioengineering, Berkeley, CA, USA.

6. Department of Molecular, Cellular and Developmental Biology, University of California, Los Angeles, CA, USA.

7. Monash Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia.

8. Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, USA.

9. Department of Earth and Planetary Sciences, University of California, Berkeley, Berkeley, CA, USA.

10. Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA.

11. Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.

Abstract

Compact defense system in bacteriophages The CRISPR-Cas system, naturally found in many prokaryotes, is widely used for genome editing. CRISPR arrays in the bacterial genome, derived from the genome of invading viruses, are used to generate a CRISPR RNA that guides the Cas enzyme to destroy repeat viral invaders. Recently, an unexpectedly compact CRISPR-Cas system was identified in huge bacteriophages. Pausch et al . show that even though this system lacks commonly found accessory proteins, it is functional. In addition to a CRISPR array, the only component of the system is an enzyme called CasF, which uses the same active site to process transcripts of the CRISPR arrays into CRISPR RNA and to destroy foreign nucleic acids. This system, which is active in human and plant cells, provides a hypercompact addition to the genome-editing toolbox. Science this issue p. 333

Funder

National Science Foundation

National Institutes of Health

Howard Hughes Medical Institute

National Multiple Sclerosis Society

William M. Keck Foundation

Paul G. Allen Frontiers Group

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference29 articles.