Programmed DNA destruction by miniature CRISPR-Cas14 enzymes-Reference-Cited by-同舟云学术

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

Published:2018-11-16 Issue:6416 Volume:362 Page:839-842
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Harrington Lucas B.¹,Burstein David²^ORCID,Chen Janice S.¹^ORCID,Paez-Espino David³,Ma Enbo¹,Witte Isaac P.¹^ORCID,Cofsky Joshua C.¹^ORCID,Kyrpides Nikos C.³^ORCID,Banfield Jillian F.²⁴⁵^ORCID,Doudna Jennifer A.¹⁵⁶⁷⁸^ORCID

Affiliation:

1. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

2. Department of Earth and Planetary Sciences, University of California, Berkeley, CA 94720, USA.

3. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA.

4. Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720, USA.

5. Innovative Genomics Institute, University of California, Berkeley, CA 94720, USA.

6. MBIB Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

7. Department of Chemistry, University of California, Berkeley, CA 94720, USA.

8. Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.

Abstract

A programmable type of CRISPR system CRISPR-Cas9 systems have been causing a revolution in biology. Harrington et al. describe the discovery and technological implementation of an additional type of CRISPR system based on an extracompact effector protein, Cas14. Metagenomics data, particularly from uncultivated samples, uncovered the CRISPR-Cas14 systems containing all the components necessary for adaptive immunity in prokaryotes. At half the size of class 2 CRISPR effectors, Cas14 appears to target single-stranded DNA without class 2 sequence restrictions. By leveraging this activity, a fast and high-fidelity nucleic acid detection system enabled detection of single-nucleotide polymorphisms. Science , this issue p. 839

Funder

National Science Foundation

U.S. Department of Energy

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference42 articles.

1. CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

2. CRISPR-Cas: Adapting to change

3. Diversity and evolution of class 2 CRISPR–Cas systems

4. The chemistry of Cas9 and its CRISPR colleagues

5. Unusual biology across a group comprising more than 15% of domain Bacteria

Cited by 843 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A novel biosensor for detecting V. parahaemolyticus based on cascade signal amplification of CRISPR/Cas14a and Exo III;Food Control;2025-01

2. DNA self-assembly-boosted transcription amplification coupled with CRISPR/Cas13a system for plant microRNA analysis;Talanta;2025-01

3. Rapid LAMP-driven strand displacement for PAM-free CRISPR-based pathogen diagnostics;Sensors and Actuators B: Chemical;2024-12

4. One-tube target amplification-free CRISPR-Cas13 autocatalytic system;Microchemical Journal;2024-11

5. Recent progress in molecular diagnostics: The synergy of rolling circle amplification and CRISPR/Cas systems (2018–2024) – A concise review;TrAC Trends in Analytical Chemistry;2024-11