Programmed DNA destruction by miniature CRISPR-Cas14 enzymes-Reference-Cited by-全球学者库

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

Published:2018-11-16 Issue:6416 Volume:362 Page:839-842
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Harrington Lucas B.¹,Burstein David²^ORCID,Chen Janice S.¹^ORCID,Paez-Espino David³,Ma Enbo¹,Witte Isaac P.¹^ORCID,Cofsky Joshua C.¹^ORCID,Kyrpides Nikos C.³^ORCID,Banfield Jillian F.²⁴⁵^ORCID,Doudna Jennifer A.¹⁵⁶⁷⁸^ORCID

Affiliation:

1. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

2. Department of Earth and Planetary Sciences, University of California, Berkeley, CA 94720, USA.

3. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA.

4. Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720, USA.

5. Innovative Genomics Institute, University of California, Berkeley, CA 94720, USA.

6. MBIB Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

7. Department of Chemistry, University of California, Berkeley, CA 94720, USA.

8. Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.

Abstract

A programmable type of CRISPR system CRISPR-Cas9 systems have been causing a revolution in biology. Harrington et al. describe the discovery and technological implementation of an additional type of CRISPR system based on an extracompact effector protein, Cas14. Metagenomics data, particularly from uncultivated samples, uncovered the CRISPR-Cas14 systems containing all the components necessary for adaptive immunity in prokaryotes. At half the size of class 2 CRISPR effectors, Cas14 appears to target single-stranded DNA without class 2 sequence restrictions. By leveraging this activity, a fast and high-fidelity nucleic acid detection system enabled detection of single-nucleotide polymorphisms. Science , this issue p. 839

Funder

National Science Foundation

U.S. Department of Energy

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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