Affiliation:
1. Department of Biology and Indiana Molecular Biology Institute, Indiana University, Bloomington, IN 47405, USA.
Abstract
In
Drosophila melanogaster
, a testis-specific β-tubulin (β2) is required for spermatogenesis. A sequence motif was identified in carboxyl termini of axonemal β-tubulins in diverse taxa. As a test of whether orthologous β-tubulins from different species are functionally equivalent, the moth
Heliothis virescens
β2 homolog was expressed in
Drosophila
testes. When coexpressed with β2, the moth isoform imposed the 16-protofilament structure characteristic of that found in the moth on the corresponding subset of
Drosophila
microtubules, which normally contain only 13-protofilament microtubules. Thus, the architecture of the microtubule cytoskeleton can be directed by a component β-tubulin.
Publisher
American Association for the Advancement of Science (AAAS)
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Cited by
125 articles.
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