Characterization of bean varieties on the basis of protein markers

Abstract

The biochemical marker phaseolin and isozymes were used in this work to display the variation of common bean germ plasma. Fifteen bean genotypes of different origin i. e. selections were studied. From 8 analyzed enzymic systems, enzymes MDH, SKDH, ME and IDH were polymorphic, while there were no differences in zymograms for enzymes PGM, PHI, PGD, and ADH. Analysis of phaseolin revealed two types: S and T. The S type of phaseolin was found in most of analyzed genotypes (9). Phaseolin type T was found in varieties of Novi Sad selection: Zlatko, Sremac and Aster, domestic population Zuto-zeleni Stepanovicevo and Jovandeka, Croatian variety Slavonski zuto-zeleni. Those varieties were developed from domestic populations from north-west region of Balkan, Slavonia, and Vojvodina.