Novel polymorphisms of dopa decarboxylase gene and their association with lamb quality traits in Indonesian sheep

Abstract

Objective: This study aimed to investigate the polymorphisms of the dopa decarboxylase (<i>DDC</i>) gene and association analysis with lamb quality and expression quantification of the <i>DDC</i> gene in phenotypically divergent Indonesian sheep.Methods: The totals of 189 rams with an average body weight of 24.12 kg at 10 to 12 months were used to identify <i>DDC</i> gene polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 189 rams, several rams representing various sheep genotypes were used for an association study between genotypes and phenotypic traits with proc general linear model (GLM) analysis. In addition, the gene expression analysis of the DDC mRNA in the phenotypically divergent sheep population was analyzed using quantitative reverse-transcription PCR.Results: The <i>DDC</i> gene (g. 5377439 G>A) showed polymorphisms that indicated three genotypes: AA, AG, and GG. The <i>DDC</i> gene polymorphism was significantly associated (p≤0.05) with carcass characteristics including carcass percentage, carcass length, hot and cold carcass; physical properties of lamb quality including pH value; retail cut carcass; fatty acid composition such as fat content, pentadecanoic acid (C15:0), tricosylic acid (C23:0), lignoceric acid (C24:0), oleic acid (C18:1n9c), elaidic acid (C18:1n9t), nervonic acid (C24:1), linoleic acid (C18:2n6c), arachidonic acid (C20:4n6), cervonic acid (C22:6n3); and mineral content including potassium (K). The GG genotype of the <i>DDC</i> gene had the best association with lamb quality traits. The <i>DDC</i> gene expression analysis mRNA showed no significant difference (p≥0.05) between lamb quality traits.Conclusion: The <i>DDC</i> gene could be used as a potential candidate gene to improve lamb quality.