Polymorphism of IGFBP7 gene (g.72351183 A>C) and its association with mineral content and cholesterol of Indonesian lamb meat

Abstract

The genetic improvement of sheep for higher productivity and meat quality is strongly needed due to the increasing demand for meat sheep. Insulin growth factor binding protein 7 (IGFBP7) has been reported as a potential gene which has a substantial role in meat quality. The objective of the study was to identify IGFBP7 gene polymorphisms and their association with minerals and cholesterol in Indonesian lamb meat. The study used 130 rams consisting of local and crossbred sheep. The local sheep were Javanese thin-tailed, Javanese fat-tailed, and Jonggol sheep. The crossbred sheep were Compass agrinak, Composite garut agrinak and Bahtera agrinak sheep. The identification of polymorphism was using PCR-RFLP method. The association analysis was obtained using a general linear model. The results showed that IGFBP7 (g.72351183 A>C) was polymorphic in local and crossbred sheep. The IGFBP7 was significantly associated with cholesterol in crossbred (P<0.01), whereas the AA genotype had the lowest cholesterol. Cholesterol in crossbred sheep also was higher (P<0.01) compared to local sheep for all observed genotypes. IGFBP7 gene was not significantly associated with minerals and cholesterol (P>0.05) in local sheep. It is concluded that IGFBP7 gene seems promising as a genetic marker for cholesterol level in Indonesian crossbred sheep.