Caveolin-1 is required for lateral line neuromast and notochord development
Author:
Nixon Susan J.123, Carter Adrian3, Wegner Jeremy4, Ferguson Charles123, Floetenmeyer Matthias123, Riches Jamie2, Key Brian3, Westerfield Monte4, Parton Robert G.123
Affiliation:
1. Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia 2. Centre for Microscopy and Microanalysis, University of Queensland, Brisbane 4072, Australia 3. School of Biomedical Sciences, University of Queensland, Brisbane 4072, Australia 4. Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA
Abstract
Caveolae have been linked to diverse cellular functions and to many disease states. In this study we have used zebrafish to examine the role of caveolin-1 and caveolae during early embryonic development. During development, expression is apparent in a number of tissues including Kupffer's vesicle, tailbud, intersomite boundaries, heart, branchial arches, pronephric ducts and periderm. Particularly strong expression is observed in the sensory organs of the lateral line, the neuromasts and in the notochord where it overlaps with expression of caveolin-3. Morpholino-mediated downregulation of Cav1α caused a dramatic inhibition of neuromast formation. Detailed ultrastructural analysis, including electron tomography of the notochord, revealed that the central regions of the notochord has the highest density of caveolae of any embryonic tissue comparable to the highest density observed in any vertebrate tissue. In addition, Cav1α downregulation caused disruption of the notochord, an effect that was enhanced further by Cav3 knockdown. These results indicate an essential role for caveolin and caveolae in this vital structural and signalling component of the embryo.
Publisher
The Company of Biologists
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