Lipid restructuring does not contribute to elevated activities of Na(+)/K(+)-ATPase in basolateral membranes from the gill of seawater-acclimated eel (Anguilla rostrata)

Abstract

In teleost fishes, increases in gill Na(+)/K(+)-ATPase activity accompanying the transition from fresh water to sea water may be attributed to changes in either the numbers of enzyme molecules present or to turnover number (k(cat)). The sensitivity of Na(+)/K(+)-ATPase to its chemical/physical environment in the membrane makes it plausible that modulation of enzyme activity may be driven, in part, by changes in membrane properties. In the current study, I test the hypothesis that lipid compositional changes (restructuring) contribute to the modulation of gill Na(+)/K(+)-ATPase activity. An enriched preparation of basolateral membranes was prepared from the gills of freshwater- and seawater-acclimated American eel (Anguilla rostrata). Phospholipid class distribution, fatty acyl chain compositions and cholesterol contents were determined. Phosphatidylcholine, the most abundant phospholipid present in gill basolateral membranes, makes up more than 60 % of the total phospholipid content in both freshwater- and seawater-acclimated animals. The contents of other phospholipids and major fatty acyl chains are also similar for the two acclimation groups. Cholesterol/phospholipid molar ratios are 0.28 for freshwater and 0.29 for seawater animals. The similarity between lipid compositions in membranes from freshwater- and seawater-acclimated eels indicates that lipid restructuring is not a mechanism for modulation of gill Na(+)/K(+)-ATPase activity in Anguilla rostrata, at least during the acclimation time course used in the present study.