Genome Editing and CRISPR/Cas System of Extremophiles and Its Applications

Abstract

Extremophiles will be the choice of next generation industrial biotechnology (NGIB) as they are known to be contaminant resistant, but engineering their genomes has always been difficult and time consuming task. CRIPR/Cas (clustered regularly interspaced short palindromic repeat and CRISPR associated proteins) system can be employed for this reason. The genome of an industrially important halophile (i.e., Halomonas) was edited to study a combined effect of four different genes on glucose breakdown and production of poly (3-hydroxybutyrate-co-3-hydroxyvalerate). This editing has resulted in 16-fold increase of 3HV, and the mutants generated by CRIPR/Cas system were significantly effective in synthesizing PHBV. Unfortunately, this system does not always work, specifically in extremophilic microorganisms because Cas9 or Cpf1 are from mesophilic bacteria. Therefore, alternatively, the endogenous CRISPR/Cas system is used for editing the genomes of such organisms. This genome editing of extremophiles will open the doors for developing next generation industrial biotechnology (NGIB).