Silver nanoparticles synthesized via green chemistry with the aid of Delphinium denudatum Wall. root extract modulated gentamicin nephrotoxicity activity with respect to oxidative potential

Abstract

Abstract Inpresent study, we explored the nephroprotective potential of Delphinium denudatum derived silver nanoparticles (DdrAgNPs). Characterisation of DdrAgNPs were carried out using UV–vis spectroscopy, X-ray diffraction (XRD), scanning electron microscope analysis (SEM), high resolution transmission electron microscopy (HR-TEM), energy dispersive x-rays (EDX) and Fourier transform infrared spectroscopy (FTIR). The biosynthesised DdrAgNPs displayed maximum absorbance at 415 nm, were polyspread in nature, sphere-shaped, and had a particle size ranging from 10 to 63 nm and the average diameter was ∼28.2 nm. Because nephroprotection could not be obtained solely through one mechanism, a considerable relationship of DdrAgNPs has been established between antioxidant potential and polyhydroxy herbal contents, suggesting that these blends may be complementary drug candidates to patients on gentamicin (GM) therapy. Biological performance of DdrAgNPs was assessed by estimating the level of renal biomarkers (blood urea, uric acid, creatinine) via antioxidants potential (GSH, CATALASE, SOD and TBARS) in experimental rats against gentamicin and corroborated with histopathological examination of renal tissue. In all, the proposed results indicate that DdrAgNPs are a potent nephroprotective agent, which can be utilised as a therapeutic adjunct to prevent gentamicin-nephrotoxicity. It is first time nephroprotection of silver nanoparticles has been reported using Delphinium denudatum as a plant extract.