Pepsin activity from gastric of milkfish and catfish in Indonesian Waters

Abstract

Abstract Pepsin is the main protease enzyme in the gastric including the gastric of the fish. The enzyme has many benefits for both food and non-food. The purpose of this study was to determined the pepsin activity of the catfish and milkfish. The study was divided into 2 stages, namely pepsin extraction stage and pepsin characterization. The results showed that pepsin from milkfish and catfish can be extracted properly using tris-Cl buffer with the activity of 526.5 U/mL (catfish) and 397 U/mL (milkfish). The pepsin isolated from the gastric of catfish and milkfish can work well at a temperature range of 20-40°C and pH 1-3. Pepsin from the gastric of catfish had Km = 0.3333 mM and Vmax = 1666.6667 mmol/s. Pepsin from milkfish has Km = 0.091 and Vmax = 909,091 mmol/s.