Improving Blood Fat Levels in Experimental Animals by Feeding on Yogurt Treated with Probiotics and Chlorella vulgaris

Abstract

Abstract The experiment included dosing experimental animals with yogurt and lacto-fermented milk supplemented fortified with probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis BB-12) and/or Chlorella vulgaris algae at a concentration of 0.6% weight/volume. The effect of treatments on blood fat levels (TG - TC - VLDL - LDL - HDL) was studied. The treatments were: Negative control (C-) fed on a standard diet, the positive control (C+) fed on rich-fat diet + 1 ml of distilled water dosed daily, or fed on rich-fat diet and dosed with 1 ml whole milk with 3% yogurt starter T1, T1 plus 5% Lactobacillus (T2), or Bifidobacteria (T3), or 0.06% Chlorella vulgaris (T4), or with 5% Lactobacillus+ 0.06 % C. vulgaris (T5), or with 5% Bifidobacteria + 0.06% C. Vulgaris (T6), or with (5% Lactobacillus+ 5% Bifidobacteria+ 0.06% C. vulgaris (T7). The results indicate that dosing with lactoferment treated with the probiotic and the alga C. vulgaris led to a significant decrease in the concentration of total cholesterol, especially in the groups synergized between the probiotic bacteria and the alga C. vulgaris, compared to the positive control groups, which recorded 113.80 mg/dl. A decrease in triglycerides was also observed in the blood of rats treated with lactofermented milk with C. vulgaris. Treatment T5 was significantly higher than the rest of the treatments, recording a decrease in triglycerides to 32.1 mg/dl. Low-density lipoproteins (LDL) also showed a significant decrease in treatments dosed with lactic fermentation with C. vulgaris, where treatment T6 was significantly superior to the rest of the treatments, recording 12.47. mg/dl. On the other hand, very low-density proteins (VLDL) recorded a significant decrease in the T4 treatment, different from the rest of the Bio-Synergy treatments, as they recorded 6.15 mg/dl. As for high-density lipoproteins, a significant increase in the coefficients of the bio-synergistic groups was observed compared to the positive control group, which recorded 15.6 mg/dl.