Determination of chymosin and bovine pepsin A activity in combined rennets on the basis of immunochemical inhibition

Abstract

SummaryThe milk clotting activity of chymosin in combined rennets was determined as the difference between the total and residual activity after specific inhibition of chymosin with the purified immunoglobulin fraction of chymosin antiserum. The milk clotting activity of bovine pepsin A was assessed in a similar manner. In bovine rennets milk clotting activity of chymosin was measured directly after immunochemical inactivation of bovine pepsin A. Any method may be used for the determination of milk clotting activity. High correlation was found between the proposed method, which is simple and rapid to perform, and Chromatographic assay.