Epidemiology ofEscherichia coliserogroups O26, O103, O111 and O145 in very young (‘bobby’) calves in the North Island, New Zealand

Author:

IRSHAD H.,COOKSON A. L.,PRATTLEY D. J.,MARSHALL J.,FRENCH N. P.

Abstract

SUMMARYThe prevalence and spatial distribution ofEscherichia coliserogroups O26, O103, O111 and O145 in calves <7 days old in New Zealand and their relationship with serum IgG, weight and sex was determined by collecting recto-anal mucosal swabs (RAMS) (n= 299) and blood samples (n= 299) from two slaughter plants in the North Island. Real-time PCR of RAMS enrichment cultures revealed that 134/299 samples were positive for O26, 68/299 for O103 and 47/299 for O145, but none were positive for O111. Processing of positive enrichment cultures resulted in 49 O26, four O103 and five O145 isolates. Using multiplex PCR 25/49 (51%) O26 isolates were positive forstx1, eae, ehxA, 17/49 (34·7%) foreae, ehxAand 7/49 (14·2%) foreaeonly. All O103 and O145 isolates were positive foreae, ehxAonly. O26 isolates were grouped into four clusters (>70% similarity) using pulsed field gel electrophoresis. Mapping of the farms showed the presence of farms positive for O26, O103 and O145 in three important dairy producing regions of the North Island. Calves positive for O103 were more likely to be positive for O26 and vice versa (P= 0·04). Similarly, calves positive for O145 were more likely to be positive for O103 and vice versa (P= 0·03). This study demonstrates that non-O157E. coliserogroups of public health and economic importance containing clinically relevant virulence factors are present in calves in the North Island of New Zealand.

Publisher

Cambridge University Press (CUP)

Subject

Infectious Diseases,Epidemiology

Reference54 articles.

1. Application of a Real-Time PCR-Based System for Monitoring of O26, O103, O111, O145 and O157 Shiga Toxin-Producing Escherichia coli in Cattle at Slaughter

2. Non‐O157 Shiga Toxin–ProducingEscherichia coliInfections in the United States, 1983–2002

3. World Health Organization. Zoonotic non-O157 Shiga toxin-producing Escherichia coli (STEC). Report of a WHO Scientific Working Group meeting (http://whqlibdoc.who.int/hq/1998/WHO_CSR_APH_98.8.pdf). 1998.

4. Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins

5. Environmental Science and Research. Monthly notifiable disease surveillance report, Wellington, 2016 (https://surv.esr.cri.nz/PDF_surveillance/MthSurvRpt/2016/201602FebRpt.pdf). Accessed 25 May 2016.

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3