Abstract
Introduction
Tuberculosis affects vulnerable groups to a greater degree, indigenous population among them.
Objective
To determine molecular epidemiology of clinical isolates of Mycobacterium tuberculosis circulating in an indigenous population through Spoligotyping and 24-loci MIRU-VNTR.
Methodology
A descriptive cross-sectional study was conducted in 23 indigenous communities of Puerto Nariño-Amazonas, Colombia. Recovered clinical isolates were genotyped. For genotyping analyzes global SITVIT2 database and the MIRU-VNTRplus web portal were used.
Results
74 clinical isolates were recovered. Genotyping of clinical isolates by spoligotyping determined 5 different genotypes, all of them belonged to Euro-American lineage. By MIRU-VNTR typing, a total of 14 different genotypes were recorded. Furthermore, polyclonal infection was found in two patients from the same community. The combination of the two methodologies determined the presence of 19 genotypes, 8 formed clusters with 63 clinical isolates in total. Based on epidemiological information, it was possible to establish a potential chain of active transmission in 10/63 (15.9%) patients.
Conclusions
High genomic homogeneity was determined in the indigenous population suggesting possible chains of active transmission. The results obtained showed that specific genotypes circulating among the indigenous population of Colombia are significantly different from those found in the general population.
Funder
Fondo de Ciencia, Tecnología e Innovación del Sistema General de Regalías BPIN
FEDER grant
Publisher
Public Library of Science (PLoS)
Reference48 articles.
1. World Health Organization. Global Tuberculosis Report 2018. Geneva. 2018
2. World Health Organization. Global Tuberculosis Report 2017. Geneva. 2017
3. Instituto Nacional de Salud. Informe Evento. Tuberculosis. Bogotá. 2016
4. Indigenous health in Latin America and the Caribbean;R Montenegro;Lancet,2006
5. Molecular epidemiology of tuberculosis and other mycobacterial infections: main methodologies and achievements;D van Soolingen;J Intern Med,2001
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