Affiliation:
1. Department of Molecular Cell Biology, University of Groningen, 9751NN Haren, Netherlands
Abstract
During embryonic development, cell movement is orchestrated by a multitude of attractants and repellents. Chemoattractants applied as a gradient, such as cAMP with Dictyostelium discoideum or fMLP with neutrophils, induce the activation of phospholipase C (PLC) and phosphoinositide 3 (PI3)-kinase at the front of the cell, leading to the localized depletion of phosphatidylinositol 4,5-bisphosphate (PI[4,5]P2) and the accumulation of phosphatidylinositol-3,4,5-trisphosphate (PI[3,4,5]P3). Using D. discoideum, we show that chemorepellent cAMP analogues induce localized inhibition of PLC, thereby reversing the polarity of PI(4,5)P2. This leads to the accumulation of PI(3,4,5)P3 at the rear of the cell, and chemotaxis occurs away from the source. We conclude that a PLC polarity switch controls the response to attractants and repellents.
Publisher
Rockefeller University Press
Cited by
46 articles.
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