Affiliation:
1. Department of Laboratory Medicine , University-Hospital of Padova , Padova , Italy
2. Department of Medicine – DIMED , University of Padova , Padova , Italy
Abstract
Abstract
Objectives
The VES-Matic 5 is an automated analyzer that assesses erythrocyte sedimentation rate based on a modified Westergren sedimentation technique. Instrument performance was established by addressing the recommendations of the International Council for Standardization in Haematology.
Methods
Comparison against the reference Westergren method was performed for all samples, and further for the low, middle, and upper third of the analytical range. Intra-run precision, inter‐run precision, and interference studies were further assessed. This study included the evaluation of reference ranges.
Results
The comparison of methods by Passing–Bablok analysis has shown a good agreement without systematic or proportional differences. The regression equation was y=−0.646 + 0.979x. The mean bias of −0.542 was obtained by Bland–Altman analysis and the upper limit of 8.03 with the lower limit of −9.11 can be considered clinically acceptable. Intra-run and inter-run precision were good for each parameter and interference studies did not show any significant bias with exception of anemia samples, which showed a proportional difference when comparing high erythrocyte sedimentation rate values. Using the local adult reference population, we verified the reference ranges in comparison to those available in the literature, and according to the Clinical Laboratory Standards Institute (CLSI) EP28-A3C document. We determined the upper limit partitioned by gender and the following age groups: from 18 to 50, from 50 to 70, and over 70.
Conclusions
The VES-Matic 5 analyzer presented good comparability with the reference method. As there are commercial quality control and suitable external quality assessment (EQA) material and programs, the VES-Matic 5 can be employed appropriately for routine purposes.
Subject
Biochemistry (medical),Clinical Biochemistry,General Medicine
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献