Stress effect on 3D culturing of MC3T3-E1 cells on microporous bovine bone slices-Reference-Cited by-同舟云学术

Stress effect on 3D culturing of MC3T3-E1 cells on microporous bovine bone slices

Published:2020-01-01 Issue:1 Volume:9 Page:1315-1325
ISSN:2191-9097
Container-title:Nanotechnology Reviews
language:en
Short-container-title:

Author:

Wang Junling¹,Zhang Yongbo²³,Yang Xiao⁴,Ma Xiaobing¹

Affiliation:

1. School of Reliability and System Engineering, Beihang University , Beijing , 100191 , China

2. Research Center of Small Sample Technology, School of Aeronautic Science and Engineering, Beihang University , Beijing , 100191 , China

3. Aircraft and Propulsion Laboratory, Ningbo Institute of Technology, Beihang University , Ningbo , 315100 , China

4. Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University , Beijing , 100191 , China

Abstract

Abstract The choosing of micromechanical environment is very important for the growth of bone-related cells. In this paper, bovine cancellous bone slices with 3D porous structures were used for 3D culturing of MC3T3-E1 cells (Mouse embryo osteoblast precursor cells) through a four-point-bending device due to their good biocompatibility and strength. Effects of micromechanical environment on the growth of MC3TC-E1 cells were investigated by immunofluorescent staining and alkaline phosphatase analysis, and the most positive microporous structures were found. In addition, a model of cell density vs stress was established through a specific normalization method and finite element simulation. The results showed that the micromechanical environment of the bone slices promoted cell proliferation, and the detail influence of stress on cell proliferation could be described by the mathematical model, which could provide a theoretical basis for the design of micromechanical environment in the bone tissue engineering scaffolds to stimulate cell proliferation.

Publisher

Walter de Gruyter GmbH

Subject

Surfaces, Coatings and Films,Process Chemistry and Technology,Energy Engineering and Power Technology,Biomaterials,Medicine (miscellaneous),Biotechnology

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