Soluble cytoplasmic expression, rapid purification, and characterization of cyanovirin-N as a His-SUMO fusion-Reference-Cited by-同舟云学术

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Soluble cytoplasmic expression, rapid purification, and characterization of cyanovirin-N as a His-SUMO fusion

Published:2009-06-23 Issue:4 Volume:85 Page:1051-1060
ISSN:0175-7598
Container-title:Applied Microbiology and Biotechnology
language:en
Short-container-title:Appl Microbiol Biotechnol

Author:

Gao Xianglei,Chen Wei,Guo Chaowan,Qian Chuiwen,Liu Ge,Ge Feng,Huang Yadong,Kitazato Kaio,Wang Yifei,Xiong Sheng

Publisher

Springer Science and Business Media LLC

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Link

http://link.springer.com/content/pdf/10.1007/s00253-009-2078-5.pdf

Reference42 articles.

1. Assadi-Porter FM, Patry S, Markley JL (2008) Efficient and rapid protein expression and purification of small high disulfide containing sweet protein brazzein in E-coli. Protein Expr Purif 58:263–268

2. Barrientos LG, O'Keefe BR, Bray M, Anthony S, Gronenborn AM, Boyd MR (2003) Cyanovirin-N binds to the viral surface glycoprotein, GP(1, 2) and inhibits infectivity of Ebola virus. Antiviral Res 58:47–56

3. Bewley CA, Gustafson KR, Boyd MR, Covell DG, Bax A, Clore GM, Gronenborn AM (1998) Solution structure of cyanovirin-N, a potent HIV-inactivating protein. Nat Struct Biol 5:571–578

4. Boyd MR, Gustafson KR, McMahon JB, Shoemaker RH, O'Keefe BR, Mori T, Gulakowski RJ, Wu L, Rivera MI, Laurencot CM, Currens MJ, Cardellina JH 2nd, Buckheit RW Jr, Nara PL, Pannell LK, Sowder RC 2nd, Henderson LE (1997) Discovery of cyanovirin-N, a novel human immunodeficiency virus-inactivating protein that binds viral surface envelope glycoprotein gp120: potential applications to microbicide development. Antimicrob Agents Chemother 41:1521–1530

5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

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1. Structural and functional analysis of Cyanovirin-N homologs: Carbohydrate binding affinities and antiviral potential of cyanobacterial peptides;Journal of Molecular Graphics and Modelling;2024-06

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4. Evaluation of Microalgae Antiviral Activity and Their Bioactive Compounds;Antibiotics;2021-06-20

5. Design of fusion protein for efficient preparation of cyanovirin-n and rapid enrichment of pseudorabies virus;Biotechnology Letters;2021-05-09

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