Design of fusion protein for efficient preparation of cyanovirin-n and rapid enrichment of pseudorabies virus
Author:
Funder
National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Subject
General Medicine,Biotechnology,Bioengineering,Applied Microbiology and Biotechnology
Link
https://link.springer.com/content/pdf/10.1007/s10529-021-03141-x.pdf
Reference12 articles.
1. Buffa V, Stieh D, Mamhood N, Hu Q, Fletcher P, Shattock RJ (2009) Cyanovirin-n potently inhibits human immunodeficiency virus type 1 infection in cellular and cervical explant models. J Gen Virol 90:234–243
2. Gao X, Chen W, Guo C, Qian C, Liu G, Ge F, Huang Y, Kitazato K, Wang Y, Xiong S (2010) Soluble cytoplasmic expression, rapid purification, and characterization of cyanovirin-N as a His-SUMO fusion. Appl Microbiol Biotechnol 85:1051–1060
3. Kleiner-Grote GRM, Risse JM, Friehs K (2018) Secretion of recombinant proteins from E. coli. Eng Life Sci 18:532–550
4. Lusvarghi S, Lohith K, Morin-Leisk J, Ghirlando R, Hinshaw JE, Bewley CA (2016) Binding site geometry and subdomain valency control effects of neutralizing lectins on hiv-1 viral particles. Acs Infectious Dis 2:882–891
5. Madeira LM, Szeto TH, Ma KC, Drake PMW (2016) Rhizosecretion improves the production of cyanovirin-n in nicotiana tabacum through simplified downstream processing. Biotechnol J 11(7):910–919
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