Efficient CRISPR/Cas9-mediated gene editing in Guangdong small-ear spotted pig cells using an optimized electrotransfection method

Author:

Wei Yan-yan,Zhan Qun-mei,Zhu Xiang-xingORCID,Yan Ai-fen,Feng Juan,Liu Lian,Li Jian-hao,Tang Dong-sheng

Funder

National Science and Technology Major Project

Guangdong Provincial R&D Project in Key Areas

Foshan Science and Technology Innovation Project

Open Project of Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding

Guangdong Foundation and Applied Basic Research Fund

Guangzhou Science and Technology Planning Project

Publisher

Springer Science and Business Media LLC

Subject

General Medicine,Biotechnology,Bioengineering,Applied Microbiology and Biotechnology

Reference34 articles.

1. Bi YZ, Hua ZD, Liu XM, Hua WJ, Ren HR, Xiao HM, Zhang LP, Li L, Wang ZR, Laible G, Wang Y, Dong FM, Zheng XM (2016) Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP. Sci Rep 6:31729. https://doi.org/10.1038/srep31729

2. Burkard C, Lillico SG, Reid E, Jackson B, Mileham AJ, Ait-Ali T, Whitelaw CB, Archibald AL (2017) Precision engineering for PRRSV resistance in pigs: macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function. PLoS Pathog 13(2):e1006206. https://doi.org/10.1371/journal.ppat.1006206

3. Gao Q, Wang M, Yu C, Huang L, Zheng X, Zhu Y (2014) Optimization of the electroporation conditions for DNA transformation of Staphylococcus carnosus. In: Zhang TC, Ouyang P, Kaplan S, Skarnes B (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251, Springer, Berlin, Heidelberg, pp 1699–1707.

4. Grobet L, Martin LJR, Poncelet D, Pirottin D, Brouwers B, Riquet J, Schoeberlein A, Dunner S, Menissier F, Massabanda J, Fries R, Hanset R, Georges M (1997) A deletion in the bovine myostatin gene causes the double–muscled phenotype in cattle. Nat Genet 17(1):71–74. https://doi.org/10.1038/ng0997-71

5. Guo H, Hao R, Wei Y, Sun D, Sun S, Zhang Z (2012) Optimization of electrotransfection conditions of mammalian cells with different biological features. J Membr Biol 245(12):789–795. https://doi.org/10.1007/s00232-012-9480-0

Cited by 7 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3