Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP-Reference-Cited by-同舟云学术

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Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP

Published:2016-08-17 Issue:1 Volume:6 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Bi Yanzhen,Hua Zaidong,Liu Ximei,Hua Wenjun,Ren Hongyan,Xiao Hongwei,Zhang Liping,Li Li,Wang Zhirui,Laible Götz,Wang Yan,Dong Faming,Zheng Xinmin

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Link

http://www.nature.com/articles/srep31729.pdf

Reference50 articles.

1. Carlson, D. F. et al. Strategies for selection marker-free swine transgenesis using the Sleeping Beauty transposon system. Transgenic Research 20, 1125–1137 (2011).

2. Yu, S. et al. Highly efficient modification of beta-lactoglobulin (BLG) gene via zinc-finger nucleases in cattle. Cell Research 11, 1638–1640 (2011).

3. Lillico, S. G. et al. Live pigs produced from genome edited zygotes. Scientific Reports 3, 2847 (2013).

4. Han, H. et al. One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system. Frontiers of Agricultural Science and Engineering 1, 2–5 (2014).

5. Xu, Y. et al. Excision of selectable genes from transgenic goat cells by a protein transducible TAT-Cre recombinase. Gene 419, 70–74 (2008).

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