Evaluation of different IRES-mediated tricistronic plasmid designs for expression of an anti-PCSK9 biosimilar monoclonal antibody in CHO cells
Author:
Funder
CAPES
Publisher
Springer Science and Business Media LLC
Subject
General Medicine,Biotechnology,Bioengineering,Applied Microbiology and Biotechnology
Link
https://link.springer.com/content/pdf/10.1007/s10529-020-02952-8.pdf
Reference22 articles.
1. Ahmadi S, Davami F, Davoudi N, Nematpour F, Ahmadi M, Ebadat S, Azadmanesh K, Barkhordari F, Mahboudi F (2017) Monoclonal antibodies expression improvement in CHO cells by PiggyBac transposition regarding vectors ratios and design. PLoS ONE 12:e0179902
2. Balasubramanian S, Rajendra Y, Baldi L, Hacker DL, Wurm FM (2016) Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines. Biotechnol Bioeng 113:1234–1243
3. Bayat H, Hossienzadeh S, Pourmaleki E, Ahani R, Rahimpour A (2018) Evaluation of different vector design strategies for the expression of recombinant monoclonal antibody in CHO cells. Prep Biochem Biotechnol 48:160–164
4. Berkowitz SA, Engen JR, Mazzeo JR, Jones GB (2012) Analytical tools for characterizing biopharmaceuticals and the implications for biosimilars. Nat Rev Drug Discov 11:527–540
5. Catapano AL, Pirillo A, Norata GD (2017) Anti-PCSK9 antibodies for the treatment of heterozygous familial hypercholesterolemia: patient selection and perspectives. Vasc Health Risk Manag 13:343–351
Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
1. Characterization of Critical Quality Attributes of an Anti-PCSK9 Monoclonal Antibody;Biologics;2024-09-11
2. Increase recombinant antibody yields through optimizing vector design and production process in CHO cells;Applied Microbiology and Biotechnology;2022-07-05
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