Monoclonal antibodies expression improvement in CHO cells by PiggyBac transposition regarding vectors ratios and design

Author:

Ahmadi Samira,Davami Fatemeh,Davoudi Noushin,Nematpour Fatemeh,Ahmadi Maryam,Ebadat Saeedeh,Azadmanesh Kayhan,Barkhordari Farzaneh,Mahboudi FereidounORCID

Funder

Pasteur Institute of Iran

Publisher

Public Library of Science (PLoS)

Subject

Multidisciplinary

Reference35 articles.

1. Control of IgG LC:HC ratio in stably transfected CHO cells and study of the impact on expression, aggregation, glycosylation and conformational stability;SC Ho;Journal of biotechnology,2013

2. Identifying bottlenecks in transient and stable production of recombinant monoclonal-antibody sequence variants in Chinese hamster ovary cells;M Mason;Biotechnology progress,2012

3. Advances in recombinant antibody manufacturing;R Kunert;Applied microbiology and biotechnology,2016

4. IgG Aggregation Mechanism for CHO Cell Lines Expressing Excess Heavy Chains;SC Ho;Molecular biotechnology,2015

5. Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells;R Haryadi;PloS one,2015

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