Increase recombinant antibody yields through optimizing vector design and production process in CHO cells-Reference-Cited by-同舟云学术

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Increase recombinant antibody yields through optimizing vector design and production process in CHO cells

Published:2022-07-05 Issue:13-16 Volume:106 Page:4963-4975
ISSN:0175-7598
Container-title:Applied Microbiology and Biotechnology
language:en
Short-container-title:Appl Microbiol Biotechnol

Author:

Yang Yongxiao,Li Zhengmei,Li Qin,Ma Kai,Lin Yan,Feng Huigen,Wang Tianyun^ORCID

Funder

Chinese National Natural Sciences Foundation

the Basic Research Project of Henan Provincial Key Scientific Research Program

Publisher

Springer Science and Business Media LLC

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Link

https://link.springer.com/content/pdf/10.1007/s00253-022-12051-5.pdf

Reference40 articles.

1. Ahmadi M, Mahboudi F, Ahmadi S, Ebadat S, Nematpour F, Akbari Eidgahi MR, Davami F (2017) PhiC31 integrase can improve the efficiency of different construct designs for monoclonal antibody expression in CHO cells. Protein Expr Purif 134:89–95. https://doi.org/10.1016/j.pep.2017.04.005

2. Ahmadi S, Davami F, Davoudi N, Nematpour F, Ahmadi M, Ebadat S, Azadmanesh K, Barkhordari F, Mahboudi F (2017) Monoclonal antibodies expression improvement in CHO cells by PiggyBac transposition regarding vectors ratios and design. PLoS ONE 12(6):e0179902. https://doi.org/10.1371/journal.pone.0179902

3. Aki Y, Katsumata Y, Kakihara H, Nonaka K, Fujiwara K (2021) 4-(2,5-Dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide improves monoclonal antibody production in a Chinese hamster ovary cell culture. PLoS ONE 16(4):e0250416. https://doi.org/10.1371/journal.pone.0250416

4. Ayyar BV, Arora S, Ravi SS (2017) Optimizing antibody expression: the nuts and bolts. Methods 116:51–62. https://doi.org/10.1016/j.ymeth.2017.01.009

5. Bayat H, Hossienzadeh S, Pourmaleki E, Ahani R, Rahimpour A (2018) Evaluation of different vector design strategies for the expression of recombinant monoclonal antibody in CHO cells. Prep Biochem Biotechnol 48(2):160–164. https://doi.org/10.1080/10826068.2017.1421966

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1. Introduction to recombinant protein expression and purification;Fundamentals of Recombinant Protein Production, Purification and Characterization;2025

2. Novel and effective screening system for recombinant protein production in CHO cells;Scientific Reports;2024-09-06

3. Enhancing recombinant antibody yield in Chinese hamster ovary cells;Tzu Chi Medical Journal;2024-05-24

4. Overexpression of YTHDF3 increases the specific productivity of the recombinant protein in CHO cells by promoting the translation process;Biotechnology Journal;2024-04

5. Synthetic enhancers including TFREs improve transgene expression in CHO cells;Heliyon;2024-03

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