Effect of Tris Buffer in the Intensity of the Multipoint Covalent Immobilization of Enzymes in Glyoxyl-Agarose Beads

Author:

Braham Sabrina Ait,Morellon-Sterling Roberto,de Andrades Diandra,Rodrigues Rafael C.,Siar El-Hocine,Aksas Ali,Pedroche Justo,Millán Maria del Carmen,Fernandez-Lafuente RobertoORCID

Funder

MINISTERIO DE CIENCIA E INNOVACION

Publisher

Springer Science and Business Media LLC

Subject

Molecular Biology,Applied Microbiology and Biotechnology,Biochemistry,General Medicine,Bioengineering,Biotechnology

Reference77 articles.

1. Bolivar, J. M., López-Gallego, F., Godoy, C., Rodrigues, D. S., Rodrigues, R. C., Batalla, P., Rocha-Martín, J., Mateo, C., Giordano, R. L. C., & Guisán, J. M. (2009). The presence of thiolated compounds allows the immobilization of enzymes on glyoxyl agarose at mild pH values: New strategies of stabilization by multipoint covalent attachment. Enzyme and Microbial Technology, 45(6–7), 477–483. https://doi.org/10.1016/j.enzmictec.2009.09.001.

2. Megías, C., Pedroche, J., Yust, M. D. M., Alaiz, M., Girón-Calle, J., Millán, F., & Vioque, J. (2006). Immobilization of angiotensin-converting enzyme on glyoxyl-agarose. Journal of Agricultural and Food Chemistry, 54(13), 4641–4645. https://doi.org/10.1021/jf0606860.

3. Bruni, M., Robescu, M. S., Ubiali, D., Marrubini, G., Vanna, R., Morasso, C., Benucci, I., Speranza, G., & Bavaro, T. (2020). Immobilization of γ-glutamyl transpeptidase from equine kidney for the synthesis of kokumi compounds. ChemCatChem, 12(1), 210–218. https://doi.org/10.1002/cctc.201901464.

4. Guerrero, C., Valdivia, F., Ubilla, C., Ramírez, N., Gómez, M., Aburto, C., Vera, C., & Illanes, A. (2019). Continuous enzymatic synthesis of lactulose in packed-bed reactor with immobilized Aspergillus oryzae β-galactosidase. Bioresource Technology, 278(October 2018), 296–302. https://doi.org/10.1016/j.biortech.2018.12.018.

5. Orrego, A. H., Romero-Fernández, M., Millán-Linares, M., Yust, M., Guisán, J., & Rocha-Martin, J. (2018). Stabilization of enzymes by multipoint covalent attachment on aldehyde-supports: 2-picoline borane as an alternative reducing agent. Catalysts, 8(8), 333. https://doi.org/10.3390/catal8080333.

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