Limitations of the Plasmid-Based Cas9-Zinc Finger Fusion System for Homology-Directed Knock-In in Chinese Hamster Ovary Cells
Author:
Publisher
Springer Science and Business Media LLC
Subject
Biomedical Engineering,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Link
https://link.springer.com/content/pdf/10.1007/s12257-022-0348-6.pdf
Reference36 articles.
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3. Lee, J. S., T. B. Kallehauge, L. E. Pedersen, and H. F. Kildegaard (2015) Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway. Sci. Rep. 5: 8572.
4. Shin, S. W. and J. S. Lee (2020) CHO cell line development and engineering via site-specific integration: challenges and opportunities. Biotechnol. Bioprocess Eng. 25: 633–645.
5. Carlson-Stevermer, J., A. A. Abdeen, L. Kohlenberg, M. Goedland, K. Molugu, M. Lou, and K. Saha (2017) Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing. Nat. Commun. 8: 1711.
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