Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/srep08572.pdf
Reference37 articles.
1. Noh, S. M., Sathyamurthy, M. & Lee, G. M. Development of recombinant Chinese hamster ovary cell lines for therapeutic protein production. Curr. Opin. Chem. Eng. 2, 391–397 (2013).
2. Wurm, F. M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 22, 1393–1398 (2004).
3. Wilson, C., Bellen, H. J. & Gehring, W. J. Position effects on eukaryotic gene expression. Annu. Rev. Cell Biol. 6, 679–714 (1990).
4. Kim, S. J., Kim, N. S., Ryu, C. J., Hong, H. J. & Lee, G. M. Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 58, 73–84 (1998).
5. Huang, Y. et al. An efficient and targeted gene integration system for high-level antibody expression. J. Immunol. Methods 322, 28–39 (2007).
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