Effect of biotic elicitor and donor NO treatment in complex defence of wheat plants against hypoxia and wounding stress

Abstract

Aim. The aim of the research was to investigate the induction of the Triticum aestivum L. non-specific tolerance by ferulic acid as a biotic elicitor and donor of nitric oxide as a signal molecule to hypoxia and wounding stress. Methods. Winter wheat plants cv. Kesariia Polis`ka were grown and had  treated by ferulic acid and sodium nitroprusside (SNP) solutions then wounding or flooding stress started. The hydrogen peroxide content was measured in leaves during the experimental period. Results. It is shown that an influence of the nitric oxide donor balanced the hydrogenous peroxide level in wounded leaves close to control. After two days of flooding stress plants treated by ferulic acid decreased hydrogen peroxide level on 15 % against threefold decreasing in non-treated stressed plants. Donor NO treatment also paid contribution to hydrogen peroxide equilibrium during the first hour under hypoxia conditions caused by flooding stress and decreased hydrogen peroxide content till the third day of stress. Conclusions. Ferulic acid and nitric oxide donor are perspective components for complex defense against hypoxia and wounding in wheat plants.