Identification of mutations related to phytate accumulation in barley kernels

Abstract

Aim. To conduct DNA marker testing on a sample of Ukrainian selection barley samples for identify various lpa-mutations that affect to the qualitative composition of phosphorus in barley grains. Мethods. Isolation of DNA (CTAB method), polymerase chain reaction (PCR) and electrophoretic determination of DNA amplification products in agarose gel for the identification of lpa mutations in barley grains. Results. The conducted molecular genetic studies of 20 breeding lines of hulless barley made possible to identify samples that have lpa-1 and lpa-2 mutations. The presence of such mutant genes determines a low content of organic (unavailable inorganic) phosphorus and an increased content of mineral (available) phosphorus that can be absorbed by the human body. Conclusions. The applied methods of identification of lpa-1 and lpa-2 mutations that affecting to the phytate content in whole grain barley allow for effective analysis of selection lines and mutant samples that can be used in future crossing schemes.