Affiliation:
1. Department of Biochemistry and Fralin Biotechnology Center, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 (S.E.B., J.K., G.E.G.); and
2. Department of Plant and Microbial Biology, University of California, Berkeley, California 94702 (W.G.)
Abstract
Abstract
The inositol triphosphate (IP3)-signaling pathway has been associated with several developmental and physiological processes in plants, but we currently know little about the regulation of this pathway. Inositol 5′ phosphatases (5PTases) are enzymes that remove a 5′ phosphate from several potential second messengers, including IP3. In catalyzing the removal of a 5′ phosphate from second messenger substrates, 5PTases can act to terminate signal transduction events. We describe the molecular analysis of At5PTase1, a 5PTase gene from Arabidopsis. When expressed transiently in Arabidopsis leaf tissue or ectopically in transgenic plants, At5PTase1 allowed for the increased hydrolysis of I(1,4,5)P3 and I(1,3,4,5)P4 substrates. At5PTase1 did not hydrolyze I(1)P, I(1,4)P2, or PI(4,5)P2 substrates. This substrate specificity was similar to that of the human Type I 5PTase. We identified 14 other potential At5PTase genes and constructed an unrooted phylogenetic tree containing putative Arabidopsis, human, and yeast 5PTase proteins. This analysis indicated that the Arabidopsis 5PTases were grouped in two separate branches of the tree. The multiplicity of At5PTases indicates that these enzymes may have different substrate specificities and play different roles in signal termination in Arabidopsis.
Publisher
Oxford University Press (OUP)
Subject
Plant Science,Genetics,Physiology
Cited by
74 articles.
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