Positional Cloning in Arabidopsis. Why It Feels Good to Have a Genome Initiative Working for You-Reference-Cited by-同舟云学术

Positional Cloning in Arabidopsis. Why It Feels Good to Have a Genome Initiative Working for You

Published:2000-07-01 Issue:3 Volume:123 Page:795-806
ISSN:1532-2548
Container-title:Plant Physiology
language:en
Short-container-title:

Author:

Lukowitz Wolfgang¹,Gillmor C. Stewart¹²,Scheible Wolf-Rüdiger¹

Affiliation:

1. Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, California 94305 (W.L., C.S.G., W.-R.S.); and

2. Department of Biological Sciences, Stanford University, Gilbert Hall, Stanford, California 94305 (C.S.G.)

Abstract

Abstract Positional (or map-based) cloning techniques are widely used to identify the protein products of genes defined by mutation. In Arabidopsis the information generated by the Genome Initiative is giving this approach a decisive boost. A wealth of sequence polymorphisms and molecular markers is now available and can be exploited for fine mapping with technically simple and robust polymerase chain reaction-based methods. As a result it has become possible to complete positional cloning projects in a short time and with relatively little effort.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

Link

http://academic.oup.com/plphys/article-pdf/123/3/795/38673705/plphys_v123_3_795.pdf

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